This protein molecular weight calculator converts a single-letter amino acid sequence into an accurate mass estimate in Daltons and kiloDaltons. Molecular biologists, biochemists, and graduate students use it daily to plan SDS-PAGE gels, set up mass spectrometry runs, and calculate molar concentrations for purified protein samples.
Accepted: single-letter IUPAC codes (A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y). FASTA headers and spaces are ignored automatically.
Each disulfide bond reduces MW by 2.016 Da (loss of 2H).
Reference: Standard Amino Acid Residue Masses
Average residue masses (Da) used in this calculator's molecular weight calculation, after loss of water during peptide bond formation.
| Code | Amino Acid | Residue Mass (Da) |
|---|---|---|
| G | Glycine | 57.0519 |
| A | Alanine | 71.0788 |
| S | Serine | 87.0782 |
| P | Proline | 97.1167 |
| V | Valine | 99.1326 |
| T | Threonine | 101.1051 |
| C | Cysteine | 103.1388 |
| L | Leucine | 113.1594 |
| I | Isoleucine | 113.1594 |
| N | Asparagine | 114.1038 |
| D | Aspartic acid | 115.0886 |
| Q | Glutamine | 128.1307 |
| K | Lysine | 128.1741 |
| E | Glutamic acid | 129.1155 |
| M | Methionine | 131.1926 |
| H | Histidine | 137.1411 |
| F | Phenylalanine | 147.1766 |
| R | Arginine | 156.1875 |
| Y | Tyrosine | 163.1760 |
| W | Tryptophan | 186.2132 |
How to Use the Protein Molecular Weight Calculator
This tool calculates the molecular weight (MW) of a protein directly from its primary amino acid sequence. It is designed for molecular biologists, biochemists, and students who need a fast, accurate estimate of protein mass without manually summing residue weights or looking up reference tables.
- Paste your protein sequence into the text box using standard single-letter amino acid codes (A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y). FASTA headers (lines beginning with
>), spaces, and line breaks are stripped automatically, so you can paste a sequence straight from a FASTA file or a database record. - Alternatively, click Upload .txt / .fasta file to load a sequence directly from a saved file rather than copying and pasting.
- If your protein contains disulfide bonds, enter the number of Cys–Cys pairs you expect to be formed. The calculator will only apply as many bonds as the cysteine count in your sequence allows.
- Choose a terminal adjustment if your protein has a known N-terminal acetylation or pyroglutamate formation; otherwise leave this set to "None."
- Click Calculate Molecular Weight. The tool returns the total MW in Daltons (Da) and kiloDaltons (kDa), the total residue count, the average residue mass, and a full breakdown of amino acid composition.
The Molecular Weight Formula
Protein molecular weight is calculated by summing the average residue mass of every amino acid in the sequence, adding the mass of one water molecule for the free N- and C-termini, then applying any disulfide bond or terminal modification corrections:
Here, residue mass is the mass remaining after each amino acid loses a water molecule during peptide bond formation; these are the standard average residue masses used across major proteomics databases. The +18.02 Da term accounts for the free hydroxyl and amine groups at the unbound termini of a linear peptide. The disulfide bond term subtracts 2.016 Da (two hydrogen atoms) for every Cys–Cys bond formed, since oxidative bond formation releases these atoms. The terminal adjustment accounts for common co- or post-translational changes at the protein ends, such as N-terminal acetylation (+42.01 Da) or pyroglutamate formation (−17.03 Da).
When to Use This Calculator
- Planning SDS-PAGE or Western blots — knowing the expected MW helps you choose the correct gel percentage and predict where your band of interest should migrate relative to your ladder.
- Setting up mass spectrometry experiments — an accurate predicted mass lets you confirm intact-protein MS results or set instrument parameters before a run.
- Calculating molar concentrations — converting a measured mg/mL protein concentration to molarity requires the MW, which is essential for setting up binding assays, enzyme kinetics, or stoichiometric reactions.
- Verifying a cloned or recombinant construct — comparing the calculated MW of your designed sequence against the observed band size is a quick sanity check after expression and purification.
Common Mistakes to Avoid
- Including FASTA headers or non-sequence text accidentally. Although this tool strips lines starting with
>automatically, stray annotation text without that symbol can be misread as amino acid codes and inflate your residue count. - Forgetting to account for tags or signal peptides. If your construct includes a His-tag, GST-tag, or an uncleaved signal/propeptide sequence, the mature protein's actual MW will differ from a calculation based on the full open reading frame.
- Double-counting or omitting disulfide bonds. Entering more disulfide bonds than your sequence has cysteine pairs available, or omitting known bonds in a heavily disulfide-bonded protein, will skew the predicted mass away from the value you would observe on a non-reducing gel or by intact mass spectrometry.
- Ignoring post-translational modifications. Glycosylation, phosphorylation, and similar modifications are not included in this calculation and can add anywhere from a few Daltons to tens of kilodaltons to the true mass of the expressed protein.
Interpreting Your Results
The molecular weight in Da and kDa represents the calculated mass of your unmodified, linear sequence under the conditions you specified (disulfide bonds and terminal adjustment). The residue count confirms how many valid amino acid characters were read from your input — if this number looks lower than expected, check the results note for any characters that were ignored as non-standard. The average residue MW (total MW divided by residue count) is a useful sanity check: values outside the roughly 100–120 Da range usually indicate an unusually biased amino acid composition or an error in the input sequence. Finally, the composition table shows the count and percentage of each amino acid, which is useful for assessing physicochemical properties such as overall hydrophobicity or charge distribution.
Frequently Asked Questions
What's the difference between average and monoisotopic molecular weight for a protein?
Average molecular weight uses the natural isotopic abundance of each element, which is the value reported by this calculator and most lab balances. Monoisotopic mass instead uses only the most abundant isotope of each atom (e.g., 12C, 1H, 16O) and is the value used in high-resolution mass spectrometry. For proteins larger than a few kDa, the difference between the two values grows because of the increasing number of atoms involved, so average mass is appropriate for general lab use while monoisotopic mass is reserved for precise MS-based identification.
Why does my calculated molecular weight differ slightly from what's listed in UniProt or a vendor certificate of analysis?
Small discrepancies usually come from differences in starting assumptions: UniProt typically reports the mass of the full-length, unmodified precursor protein, which may include a signal peptide or propeptide that is cleaved in the mature, functional form. Vendor certificates often reflect the mature, processed protein and may also account for tags, glycosylation, or other post-translational modifications that this calculator does not include. Rounding conventions and the use of monoisotopic versus average masses can also account for differences of a few Daltons.
How do disulfide bonds affect the calculated molecular weight?
Each disulfide bond forms when two cysteine residues are oxidized together, releasing two hydrogen atoms and reducing the total mass by 2.016 Da per bond. This calculator automatically subtracts this amount for every disulfide bond you specify, up to the maximum number possible given the number of cysteine residues in your sequence. If your protein's folding state or expression system is uncertain, it's safest to calculate molecular weight both with and without disulfide bonds applied so you can interpret SDS-PAGE bands run under both reducing and non-reducing conditions.
Can this tool calculate the molecular weight of a protein with post-translational modifications?
No, this calculator computes molecular weight directly from the primary amino acid sequence and only accounts for free N- and C-termini, disulfide bonds, and a small set of terminal modifications. Modifications such as glycosylation, phosphorylation, ubiquitination, or proteolytic cleavage are not included and can add anywhere from a few Daltons to tens of kDa depending on the modification. If your protein carries known modifications, add their combined mass manually to the unmodified value reported here.
What amino acid residue mass values does this calculator use, and how accurate are they?
The calculator uses the standard average residue masses for the 20 canonical amino acids, derived from the average atomic weights of their constituent atoms after loss of water during peptide bond formation. These values match those used by major proteomics resources such as ExPASy and the NCBI, so results for unmodified, linear sequences are accurate to within a fraction of a Dalton. Accuracy decreases only when the sequence contains non-standard residues, modified amino acids, or characters outside the 20 standard single-letter codes, which this tool flags and excludes from the calculation.