About PCR Tools
PCR (Polymerase Chain Reaction) is one of the most fundamental techniques in molecular biology. These tools help you design optimal primers, calculate annealing temperatures, set up reaction mixtures and troubleshoot your PCR experiments.
All tools work directly in your browser. No data is sent to any server. Your sequences remain completely private and secure.
All 14 PCR Tools
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Primer Tm Calculator
Calculate melting temperature of any primer using Wallace rule and nearest neighbour method.
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Primer GC Content Calculator
Calculate GC percentage of any primer sequence with quality assessment for PCR suitability.
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Annealing Temperature Calculator
Calculate optimal PCR annealing temperature from forward and reverse primer Tm values.
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PCR Master Mix Calculator
Calculate exact volumes for complete PCR reaction setup. Supports multiple reaction scaling.
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Primer Length Checker
Check if your primer length is optimal for PCR. Analyzes length, composition and quality score.
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PCR Product Size Calculator
Calculate expected PCR band size from primer positions on template. Useful for gel analysis.
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Gradient PCR Temperature Calculator
Generate a temperature gradient range for PCR optimization. Helps find ideal annealing temperature.
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Primer Dimer Checker
Check for self-complementarity and potential primer dimer formation in your primer sequence.
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Primer Quality Analyzer
Complete primer quality analysis — Tm, GC%, length, 3' end, hairpin and dimer check in one tool.
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qPCR Efficiency Calculator
Calculate PCR amplification efficiency from standard curve data. Shows R² and efficiency percentage.
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RT-PCR Calculator
Calculate reaction volumes for reverse transcription PCR. Supports one-step and two-step RT-PCR.
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Colony PCR Calculator
Calculate reaction volumes for colony screening PCR. Optimized for bacterial colony templates.
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Hot Start PCR Calculator
Calculate volumes for hot start PCR reactions. Reduces non-specific amplification in complex templates.
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Multiplex PCR Tm Calculator
Calculate compatible annealing temperatures for multiple primer pairs in a single multiplex PCR reaction.
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