How to Use the RNA to DNA Converter
Step 1: Type or paste your RNA sequence in the input box above. The sequence should be written in the 5' to 3' direction.
Step 2: The tool accepts only valid RNA bases: A (Adenine), U (Uracil), G (Guanine), C (Cytosine). All spaces, numbers and other characters are automatically removed.
Step 3: Click the Convert to DNA button. Your DNA sequence will appear instantly with the thymine bases highlighted in blue.
Step 4: Use the Copy button to copy your result or Upload File to load a sequence from a .txt or .fasta file.
How RNA to DNA Conversion Works
Back transcription is the process of converting an RNA sequence back to its equivalent DNA sequence. This is the reverse of transcription and is also the basis of reverse transcriptase enzyme used in RT-PCR and retrovirus replication.
A (Adenine) → A (Adenine)
U (Uracil) → T (Thymine) ← only change
G (Guanine) → G (Guanine)
C (Cytosine) → C (Cytosine)
The only difference between RNA and DNA sequences is that DNA uses Thymine (T) instead of Uracil (U). All other bases remain the same.
Worked Example
Important Notes
This tool performs simple base substitution converting U to T. In biological reverse transcription, reverse transcriptase enzyme synthesizes a complementary DNA strand from the RNA template. This tool is useful for converting known mRNA sequences back to their coding DNA equivalent.