🔄 RT-PCR Calculator

RT-PCR Calculator

Calculate exact reagent volumes for reverse transcription PCR. Supports both one-step and two-step RT-PCR protocols with adjustable RNA input and reaction scale.

🔄 RT-PCR Calculator FREE TOOL

Protocol Type

RT Enzyme Preset

RNA Input

RNA Concentration (ng/µL) optional

RNA Amount Needed (ng) optional

RNA Input Volume (µL) ↑ auto-calculated or enter manually

Total RT Reaction Volume (µL)

Reagent Volumes (per reaction)

RT Buffer 5X (µL)

dNTPs 10mM (µL)

Primer Type

Primer Volume (µL)

Reverse Transcriptase (µL)

RNase Inhibitor (µL) optional

Reaction Setup

Number of Reactions

Extra Volume Buffer

cDNA Dilution for PCR (Two-Step only)

cDNA Dilution Factor

cDNA Volume in PCR (µL)

REACTIONS

µL PER REACTION

µL TOTAL MIX

📋 Master Mix Recipe

🧬 cDNA Dilution & PCR Setup

🌡️ Recommended Thermocycler Program