How to Use the Common Lab Buffer Recipes
Search: Type a buffer name (e.g. PBS, TAE, Tris) or ingredient (e.g. NaCl, EDTA) to filter the list instantly.
Filter by use: Use the category buttons to show only gel electrophoresis buffers, cell biology buffers, protein work buffers, or general-purpose buffers.
Expand recipe: Click any buffer card to reveal the full ingredient list and step-by-step preparation protocol.
About Laboratory Buffers
Buffers are solutions that resist changes in pH when small amounts of acid or base are added. In molecular biology and biochemistry, maintaining precise pH is critical for enzyme activity, protein stability, DNA integrity, and electrophoresis quality.
Tips for Buffer Preparation
Always use high-quality reagents (molecular biology grade or better). Prepare all buffers in ultrapure (MilliQ or equivalent) water. Adjust pH at room temperature using a calibrated pH meter with appropriate electrodes. Autoclave or filter-sterilize buffers intended for cell culture or sensitive applications. Label all buffers with name, concentration, date prepared, and initials.
Storage Guidelines
Most buffers are stable at 4Ā°C for 1ā6 months. Phosphate-buffered saline (PBS) and Tris buffers are generally stable for 6 months at room temperature if sterile. Buffers containing reducing agents (DTT, Ī²-ME) should be prepared fresh or stored at ā20Ā°C. SDS-containing buffers should be stored at room temperature to prevent precipitation.